Immunology and Infectious Diseases

Shirin Tarafder has completed her Bachelor of Medicine and Bachelor of Surgery at the age of 24 years from Dhaka University and Master of Philosophy in Medical Microbiology from Institute of Postgraduate Medicine and Research, Bangladesh. She is associate professor in the department of Microbiology and Immunology, Bangabandhu sheikh Mujib Medical University, Bangladesh. She has published 23 papers in reputed journals. She is life member of Bangladesh Society of Medical Microbiologist and Bangladesh Society of Pathologist.

Introduction: Chronic Lymphocytic Leukaemia (CLL) is a clonal B-cell neoplasm of mature cells whose diagnosis is based on clinical manifestations, cell morphology and Immunophenotyping. It is generally accepted that any lymphocytosis superior to 5000/cmm in an adult person and without any other evident cause , must raise the suspicion of a CLL diagnosis. The most reliable methodology for the diagnosis of CLL is Immunophenotyping by Flow Cytometric.

 

Objective: The aim of this study was to evaluate the application of multipara metric Flow Cytometry Immunophenotyping (FCI) as a standard methodology for the confirmation of or exclusion of CLL diagnosis in clinically suspected CLL patients.

 

Patients and Methods: Four colour flow cytometry multipara metric immunophenotyping method was used in EDTA peripheral blood samples taken from 25 consecutive patients diagnosed preliminary as CLL through clinical data, complete blood count, peripheral blood film and bone marrow examination. The following fluorescent monoclonal antibodies were used: CD19, CD5, CD20, CD22, CD23, CD79b, CD79a, FMC7, Kappa and Lambda

light chains, CD10, CD11c, CD3, CD7, CD25, CD30, CD56, CD95, BCL2, and CD34. Marker of immaturity CD34 was added to exclude blast cells. The flow cytometric analysis was performed on a Beckman coulter cytomics FC500 flow cytometer using software CXP to analyze data. Appropriate isotype control studies to determine background fluorescence were also used.